gephryin antibody (Synaptic Systems)
Synaptic Systems is a verified supplier 
Synaptic Systems manufactures this product 
94
Structured Review
Synaptic Systems
gephryin antibody
Gephryin Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gephryin+antibody/147+318/pmc10627507-5-0-3
Average 94 stars, based on 23 article reviews
Gephryin Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gephryin+antibody/147+318/pmc10627507-5-0-3
Average 94 stars, based on 23 article reviews
gephryin antibody - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species"
Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species
Journal: Cell reports
doi: 10.1016/j.celrep.2023.113151
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Virus, Recombinant, Transfection, Magnetic Beads, Luciferase, RNAscope, Multiplex Assay, Reverse Transcription, Control, Plasmid Preparation, Software
Related Articles
Virus:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Recombinant:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Transfection:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Magnetic Beads:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Luciferase:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: RNAscope:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Multiplex Assay:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Reverse Transcription:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Control:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Plasmid Preparation:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: Software:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: RNA Sequencing:Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MAP2 antibody Sigma-Aldrich Cat. # M1406; RRID:AB_477171 AlexaFluor 555 conjugated secondary antibody Thermo Fisher Cat. # A31570; RRID:AB_2536180 Anti-Synapsin I antibody Abcam Cat. # ab64581; RRID:AB_1281135 Anti-PSD95 antibody Sigma-Aldrich Cat. # P246; RRID:AB_260911 Gephryin antibody Synaptic Systems Cat. # 147 318; RRID:AB_2661777 Arc (C-7) antibody Santa Cruz Biotech Cat. # sc-17839; RRID:AB_626696 Egr1 Antibody Thermo Scientific Cat. # MA5-15009; RRID:AB_10982091 Anti-Mouse IgG (H+L), highly cross-adsorbed, CF 647 antibody Sigma-Aldrich Cat. # SAB4600176; RRID:AB_3064834 Anti-Rabbit IgG (H+L), highly cross-adsorbed, CF 555 antibody Sigma-Aldrich Cat. # SAB4600061; RRID:AB_3064836 Alexa Fluor 647-AffiniPure Donkey Anti-Guinea Pig IgG (H+L) Jackson ImmunoResearch Cat. # 706-605-148; RRID:AB_2340476 Bacterial and virus strains OP50 E. Coli (BactoBeads) Sigma-Aldrich Cat. #S29021 HT115 E coli Caenorhabditis Genetics Center HT115 Chemicals, peptides, and recombinant proteins 2-butanone Acros Organics Cat. # 332828-25ML Benzaldehyde Millipore Sigma Cat. # B1334-100G Lipofectamine 3000 Transfection Reagent Thermo Fisher Cat. # L3000008 Sigma Protector RNase inhibitor Sigma-Aldrich Cat. # 3335402001 Molecular Probes Hoechst 33342 Thermo Fisher Cat. # H3570 Ampure XP magnetic beads Beckman Coulter Cat. # A63882 Critical commercial assays Bright-Glo Luciferase Assay System Promega Cat. # E2620 RNAScope Multiplex Fluorescent Reagent Kit V2 ACD Bio Cat. # 323100 RNA-Protein Co-Detection Ancillary Kit ACD Bio Cat. # 323180 PureLink RNA Mini kit Thermo Fisher Cat. # 12183025 HighCapacity cDNA Reverse Transcription kit Thermo Fisher Cat. # 4374966 KAPA SYBR Fast mix Roche Cat. # KK4601 Papain dissociation system Worthington Cat. # LK003153 10X Genomics Chromium X system using the Single Cell 3’ v3.1 Reagent Kits 10X Genomics Cat. # 1000 Illumina Tagment DNA Enzyme and Buffer kit Illumina Cat. # 20034198 Deposited data RNA-seq data This paper BioProject #PRJNA972702. Article Title: Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4 Article Snippet: The following antibodies were diluted in TBST (0.2% Tween-20), with 2% BSA: Lrp4 (1:2500; Neuromab, N207/27, Davis, CA), Synaptophysin (1:20,000; Life Technologies, Grand Island, NY), PSD95 (1:1000; Neuromab, K28/43), GluA1 (1:1000; Millipore, Billerica, MA), GluA2/3 (1:500; Millipore), pan-Shank (1:1000; Santa Cruz Biotechnology, C-20, Dallas, TX), NR1 (1:1000; Neuromab, N308/48), GABA A R γ2 (1:1000; PhosphoSolutions, Aurora, CO), NeuN (1:1000; Millipore), Article Title: Neuronal activation of G αq EGL-30/GNAQ late in life rejuvenates cognition across species Article Snippet: |
